Experiment determine vitamin c in pineapple
Determination of vitamin c in tropical fruits: a comparative evaluation of methods yurena hernández, m gloria lobo, mónica gonzález plant physiology laboratory, department of tropical fruits, instituto canario de investigaciones. Counts of both treated and control pineapple juices were obtained during the period of monitoring but the total viable bacterial count ranged from 38 × 10 4 to 44 × 10 8 cfu/ml while the fungal count ranged from 30 × 10 4 to 52 × 10 8 cfu/ml. Experiment determine vitamin c in pineapple juice pineapple juice, 0 1% ascorbic acid solution, 2,6-dichlorophenol – indophenol (dcpip) solution, distill water, filter paper apparatus : small test tube and rack, 1cm3 and 5cm3 syringes with needles, 250ml beaker, 25ml measuring cylinder, stirrer, electronic scale. Ascorbic acid (vitamin c) is a water-soluble vitamin which can be found in many biological systems and foodstuffs (fresh vegetables and fruits, namely, citrus) ascorbic acid plays an important role in collagen biosynthesis, iron absorption, and immune response activation and is involved in wound.
The vitamin c content of over 50 commercial and wild fruit is listed, both by serving and by 100 mg sample each fruit is ranked into exceptional, excellent, very good, good, or fairly good source of ascorbic acid. The amount of vitamin c content of some fresh fruit degradaded with time was investigated pineapple, orange, watermelon and tomato stored under difference conditions the juices from the samples. And vitamin c content of pawpaw juice, pineapple juice and watermelon juice respectively 11 aim of the research the aim of the research is to find out the suitable 24 vitamin c determination 2141 method iodine titration method  2142 titation 50cm 3 un-concentrated juice was pipette into. Determination of vitamin c (ascorbic acid) vitamin c (ascorbic acid) was determined by the titrimetric method as described by mazumdar and majumder  and james [22.
A simple and rapid screening test to determine the real amount of vitamin c contained in fruit juices daily products was established by prussian blue the ferric chloride was reduced by vitamin c and then reacted with potassium ferricyanide to form a blue solution of prussian blue the developed color was monitored at absorption wavelength of 709 nm. Vitamin c (ascorbic acid) is an antioxidant that is essential for human nutrition vitamin c deficiency can lead to a disease called scurvy, which is characterized by abnormalities in the bones and teeth. Determination of vitamin c in fresh fruits and vegetables using the dye-titration and microfluorometric methods es tee, si young, sk ho and s siti mizura division ofhuman nutrition, institute for medical research, kuala lumpur, malaysia were compared using t-test reproducibility stu. 2 vitamin c (compound i) is a colorless, water-soluble acid (k a = 67 x 10-5, oneof the enolic protons is lost) and also a powerful biochemical reducing agent that readily undergoes air oxidation to. You could first use some sampled amount of vitamin c tablets, do the experiment with dcpip solutions and map out the standard curve of vitamin c, x-axis: mg/l, y-axis: drops of vitamin c solution needed.
Published: mon, 5 dec 2016 the experiment was tested with titrations being done each week of the pineapple juice which was stored in various conditions results were recorded and compared to previous week’s results in order to determine whether there is in fact deterioration due to storage of vitamin c. Please read either from hands on pg 55 /the sample experiment plan given below : objective to determine the vitamin c content in pineapple, lime and orange juice. Abstract: studying the magnitude and stability of the ascorbic acid content of commercial orange juices students were interested in ascertaining if orange juice represents a suitable source of vitamin c after one or two weeks in the refrigerator.
Redox titration of vitamin c in this experiment, we will determine the amount of ascorbic acid (the active ingredient) in commercial vitamin c tablets you may bring your own vitamin tablets from home if you wish, but be forewarned that the quality of the results can vary depending on the formulation. Test the filtrate with h&s to determine whether all the hg has been removed after standing overnight, remove the h&s by bubbling nitrogen gas through this takes about 15 minutes pineapple juice, 050 canned 054 onion eggplant peas, fresh1 042 “ “ vitamin c content of normal rabbit and guinea pig tissues. A test method must be able to assay ascorbic acid and single-strength pineapple juice single-strength orange juice cranberry juice concentrate ber of lc-uv studies for the determination of vitamin c in fruits and vegetables (22, 25, 30, 32–35), often concurrent.
Experiment determine vitamin c in pineapple
The true data state that the content of vitamin c mg per 100 ml is 15 mg but in the experiment is 08096 mg / 100 ml this is because the vitamin c is decompose by heat the diluted sample is prepared too earlier. Vitamin c plays an important role in the growth and repair of body tissues including repairing maintaining cartilage, bones and teeth, healing wounds and forming scar tissue, and aids in making skin, tendons, ligaments and blood vessels. This experiment examined the effect of vitamin c content in fruit juice on dcpip’s colour changes using acid-based titration method a titration is a method of analysis that will allow us to determine the precise endpoint of a reaction and therefore the precise quantity of reactant in the titration flask.
- Comparative analysis of vitamin c in fresh fruits juice of 19 orange is a hybrid, possibly between pomelo (citrus maxima) and mandarin (citrus reticulata), which has been cultivated since ancient times (nicolosi et al, 2000) pineapple (ananas comosus) is a tropical plant with edible multiple fruit consisting of.
- In this experiment the student will use a lab procedure known as a titration to determine the amount of vitamin c found in a 6 ounce serving of various fruit juices i most often use orange juice, pineapple juice, and apple juice.
Use titration to determine how much vitamin c there is in a fruit drink hands-on practical activities or ideas for front of class demonstrations hands-on practical activities or ideas for front of class demonstrations assessment for learning chemistry: what fruit contains the most vitamin c what. Therefore, in this experiment, the amount of vitamin c was determined as the sum of ascorbic and dehydroascorbic acids however, it is reported that the average amount of dehydroascorbic acid in commercial orange juice ranges from 0 to 02. Determination of vitamin c in a fruit juice or food each group will run two sets of titrations, one with fresh fruit juice and refrigerated juice (eg, juice from an orange and packaged orange juice) or one with a fresh vegetable and one with frozen vegetable (eg. Measure and determine the volume of standard vitamin c solution needed to decolourise of a fixed volume of dcpip measure and determine the volume of juice needed to decolourise the same volume of dcpip.